Benzo Nuclease

Benzo Nuclease is a kind of no-specific endonuclease. The Benzo Nuclease genetically engineered endonuclease from Serratia marcescens.

Benzo Nuclease degrades all kinds of DNA and RNA (double stranded, single stranded, linear and circular and supercoil) but without proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity.

Product Description

  • Description
    Benzo Nuclease completely digests nucleic acids to 5’monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzo Nuclease to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts. The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by>150 mM monovalent cations,>100 mM phosphate,>100 mM ammonium sulfate, or>100 mM guanidine HCl.
  • Storage Buffer
    20 mM Tris-Cl(pH 8. 0), 2 mM MgCl2, 2 mM NaCl, 50%Glycerin
  • Dilution Buffer
    20 mM Tris-Cl(pH 8. 0), 2 mM MgCl2, 2 mM NaCl.
  • Unit definition
    One unit is defined as the amount of enzyme that causes a ΔA260 of 1. 0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
  • Shipping and Handling
    Liquid enzyme: Ship with Blue Ice, Storage at -20°C
    Lyophilized powder: Ship at room temperature, storage blow 4°C
  • Guarantee
    2 years

Specification

  • Source: Serratia marcescens
  • Molecular weight: 27.9KD, (SDS)
  • Purity: ≥90%, (Ultra≥99%)(SDS-PAGE)
  • PI: 6.85
  • Optimum pH: 8.0
  • Optimum temperature: 37°C
  • Cofactor: 1~10mM Mg2+
  • Form: Liquid enzyme is the clear Solution(Contain Glycerin)
  • Solid enzyme is the white lyophilized powder.
  • Concentration: 1000U/µl.
  • Activity: ≥1000U/μl
  • Specific activity: ≥1.0x10^6U/mg protein
  • Protease: Not detectable
  • Microbial limit: aerobe<10CFU/100KU Yeast and mold: <10CFU/100KU
  • Endotoxin test: (Gel Clot LAL Assay)<0.25EU/1000U
  • CAS: 9025-65-4
  • EC: 3.1.30.2
Benzonase Nuclease GMP Grade, Recombinant Benzo Nuclease, Universal Nuclease, Benzonase endonuclease

Benzo Nuclease

Application

Its high intrinsic activity and broad substrate tolerance make the endonuclease an ideal tool in a variety of biotechnological and pharmaceutical applications

Removal of nucleic acid from protein samples

Elimination of nucleic acids from recombinant proteins

Purification of protein fragments from inclusion bodies

Sample preparation in western blotting or two-dimensional gel electrophoresis

Viscosity reduction in protein extracts

Order

Benzonase Nuclease, Recombinant Benzo Nuclease, Universal Nuclease, Benzonase endonuclease No-specific endonuclease, 250U/µl, 50KU/vial

Benzonase Nuclease, Lyophilized Powder, Recombinant Benzo Nuclease, Universal Nuclease, Benzonase endonuclease No-specific endonuclease, Lyophilized Powder, 50KU/bottle

Benzonase Nuclease, Lyophilized Powder, Recombinant Benzo Nuclease, Universal Nuclease, Benzonase endonuclease No-specific endonuclease, Lyophilized Powder, 100KU/bottle

Benzonase, GMP Grade, Recombinant Benzo Nuclease, Universal Nuclease, Benzonase endonuclease No-specific endonuclease, 250U/µl, 250KU/vial

Benzonase, GMP Grade, Recombinant Benzo Nuclease, Universal Nuclease, Benzonase endonuclease No-specific endonuclease, 250U/µl, 500KU/vial

Contacts

Tinzyme Co., Limited

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